研究方向：

乙型肝炎病毒（HBV）感染，特别是慢性感染，可引起慢性乙型肝炎、肝硬化和肝癌等严重后果，是重大的公共卫生问题，2016年世界卫生组织提出了“2030年消除病毒性肝炎的威胁”的目标。目前HBV感染可预防、可诊断、可治疗，但慢性感染难以彻底治愈。

HBV是易变异的病毒，我们以HBV变异及其生物学和临床意义为研究方向，应用病毒学、分子生物学和免疫学等技术方法，结合临床病例，开展基础和临床相结合的研究。从临床标本分析入手，发现和鉴定有意义的病毒突变类型，并在体外研究突变株对病毒生物学特性、耐药性、致病性、免疫逃逸等特性的影响和机制，已发表一系列相关研究论文，曾获得秦皇岛市科技进步一等奖、河北省科学技术进步奖三等奖、北京大学JDB电子官方网站SCI论文引用奖和优秀SCI论文奖等奖励。

 

在研科研课题：

本人曾承担和参与多项国家级科研课题，目前在研课题为：

    （1）国自然面上项目“揭示乙型肝炎表面抗原新突变对病毒复制的影响及突变株传播的新途径”的课题负责人；

    （2）十三五传染病重大专项课题“乙型肝炎临床治愈及疾病进展相关的新生物标志物及预测模型建立”任务负责人；

    （3）十三五传染病重大专项课题“艾滋病疫苗研究”任务负责人。

 

代表性论文：

1. Hao R, Xiang K, Shi Y, Zhao D, Tian H, Xu B, Zhu Y, Dong H, Ding H, Zhuang H, Hu Jand Li T. Naturally occurring mutations within HBV surface promoter II sequences affect transcription activity, HBsAg and HBV DNA levels in HBeAg-positive chronic hepatitis B patients. Viruses. 2019, 11(1): 78.  (co-corresponding author)

2. Xiang K, Xiao Y, Li Y, He L, Wang L, Zhuang H and Li T. The effect of the hepatitis B virus surface protein truncated sC69？mutation on viral infectivity and the host innate immune response. Front Microbiol. 2019, 10:1341. (co-corresponding author)

3. He L, Su M, Ou G, Wang L, Deng J, Zhuang H, Xiang K and Li T. The modulation of HBsAg level by sI126T is affected by additional amino acid substitutions in the S region of HBV. Infect Genet Evol. 2019, 75:104006. (co-corresponding author)

4. Xiang K, Zhao C, Wang S, Li Y, Su M, Wang Q, Xu X, Deng J, Zhuang H and Li T. Impacts of HBV rtH55R polymerase substitution on viral replication and rtM204I/V resistance to nucleoside/nucleotide antiviral drugs. Antivir Ther. 2018, 23(1): 33-42. (corresponding author)

5. Liu B, Yang J, Yan L, Zhuang H and Li T. Novel HBV recombinants between genotypes B and C in 3'-terminal reverse transcriptase (RT) sequences are associated with enhanced viral DNA load, higher RT point mutation rates and place of birth among Chinese patients. Infect Genet Evol. 2018, 57: 26-35. (co-corresponding author)

6. Li Y, He L, Li Y, Su M, Su J, Hou J, Deng J, Wang S, Wang Q, Xu X, Zhuang H and Li T. Characterization of serum HBV RNA in patients with untreated HBeAg-positive and -negative chronic hepatitis B infection. Hepatitis Monthly. 2018, 18(2):e62079. (co-corresponding author)

7. Su M, Liao L, Xing H, Wang S, Li Y, Lu W, He L, Deng J, Shao Y, Li T and Zhuang H. Characteristics of HBV infection in 705 HIV-infected patients under lamivudine-based antiretroviral treatment from three regions in China. Infect Drug Resist. 2018, 11: 1635-1644. (co-corresponding author)

8. Peng Y, Li Y, Hou J, Sun J, Su M, Li Y, Xiang K, Yan L, Zhuang H and Li T. The nucleotide changes within HBV core promoter/precore during the first 12 weeks of nucleos(t)ide treatment might be associated with a better virological response. Infect Genet Evol. 2017, 49: 116-121. (corresponding author)

9. Xiang KH, Michailidis E, Ding H, Peng YQ, Su MZ, Li Y, Liu XE, Dao Thi VL, Wu XF, Schneider WM, Rice CM, Zhuang H, Li T. Effects of amino acid substitutions in hepatitis B virus surface protein on virion secretion, antigenicity, HBsAg and viral DNA. J Hepatol. 2017. 66(2): p. 288-296. (co-corresponding author)

10. Xu X, Xiang K, Su M, Li Y, Ji W, Li Y, Zhuang H and Li T. HBV drug resistance substitutions existed before the clinical approval of nucleos(t)ide analogues: a bioinformatic analysis by GenBank data mining. Viruses. 2017, 9(1998): 199. (co-corresponding author)

11. Su M, Xiang K, Li Y, Li Y, Deng J, Xu X, Yan L, Zhuang H, Li T. Higher detection rates of amino acid substitutions in HBV reverse transcriptase/surface protein overlapping sequence is correlated with lower serum HBV DNA and HBsAg levels in HBeAg-positive chronic hepatitis B patients with subgenotype B2. Infect Genet Evol. 2016, 40:275-81. (co-corresponding author)

12. Peng Y, Liu B, Hou J, Sun J, Hao R, Xiang K, Yan L, Zhang J, Zhuang H, Li T. Naturally occurring deletions/insertions in HBV core promoter tend to decrease in hepatitis B e antigen-positive chronic hepatitis B patients during antiviral therapy. Antivir Ther. 2015, 20(6):623-32. (co-corresponding author)

13. Hao R, Xiang K, Peng Y, Hou J, Sun J, Li Y, Su M, Yan L, Zhuang H, Li T. Naturally occurring deletion/insertion mutations within HBV whole genome sequences in HBeAg-positive chronic hepatitis B patients are correlated with baseline serum HBsAg and HBeAg levels and might predict a shorter interval to HBeAg loss and seroconversion during antiviral treatment. Infect Genet Evol. 2015, 33:261-8. (co-corresponding author)

14. Ding H, Liu B, Zhao C, Yang J, Yan C, Yan L, Zhuang H,Li T. Amino acid similarities and divergences in the small surface proteins of genotype C hepatitis B viruses between nucleos(t)ide analogue-naive and lamivudine-treated patients with chronic hepatitis B. Antiviral Res. 2014. 102: 29-34. (corresponding author)

15. Yan CH, Zhao CY, Ding H, Peng YQ, Jin PY, Yan L, Zhuang H, Li T. Hepatitis B virus basal core promoter mutations A1762T/G1764A are associated with genotype C and a low serum HBsAg level in chronically-infected HBeAg-positive Chinese patients. Antiviral Res. 2012, 96(2):108-14. (co-corresponding author)

16. Li XG, Liu BM, Xu J, Liu XE, Ding H, Li T. Discrepancy of potential antiviral resistance mutation profiles within the HBV reverse transcriptase between nucleos(t)ide analogue-untreated and -treated patients with chronic hepatitis B in a hospital in China. J Med Virol. 2012, 84(2):207-16. (co-corresponding author)

17. Yang JX, Liu BM, Li XG, Yan CH, Xu J, Sun XW, Wang YH, Jiao XJ, Yan L, Dong JP, Hou CS, Abuduheilili X, Li T, Zhuang H. Profile of antiviral resistance mutations within HBV reverse transcriptase in distinct evolutionary pathways refractory to nucleos(t)ide analogue treatment among Chinese patients with chronic HBV infection. Antiv Ther. 2010, 15(8): 1171-8. (co-corresponding author)

18. Liu BM, Li T, Xu J, Li XG, Dong JP, Yan P, Yang JX, Yan L, Gao ZY, Li WP, Sun XW, Wang YH, Jiao XJ, Hou CS, Zhuang H. Characterization of potential antiviral resistance mutations in hepatitis B virus reverse transcriptase sequences in treatment na？ve Chinese patients. Antivir Res. 2010, 85(3):512-9. (co-corresponding author)

19. Du H, Li T, Zhang HY, He ZP, Dong QM, Duan XZ, Zhuang H. Correlation of hepatitis B virus (HBV) genotypes and mutations in basal core promoter/precore with clinical features of chronic HBV infection. Liver Int. 2007, 27(2):240-6. (co-first author)

 

个人其它信息：

本科毕业于北京农业大学，硕士毕业于清华大学，博士毕业于瑞典Ume？大学。从进入大学起，从未离开过微生物学领域，从事过农业、环境、工业和医学微生物学研究工作。是北京微生物学会第八、九和十届理事，第二届中国微生物学会临床微生物学专业委员会流行病学组委员，中联肝健康促进中心理事。担任Journal of Medical Virology、Journal of Virological Methods、Antiviral Therapy、Antiviral Research、Journal of Viral Hepatitis、Infection Genetics and Evolution、Infection and Drug Resistance等国际学术期刊和多家中文学术期刊的同行评审专家。